Evaluation of an automated immunomagnetic separation method for the rapid detection of Salmonella species in poultry environmental samples

Michael J B Lynch; Carlos G Leon-Velarde; Scott McEwen; Joseph A Odumeru

doi:10.1016/j.mimet.2004.04.005

Evaluation of an automated immunomagnetic separation method for the rapid detection of Salmonella species in poultry environmental samples

J Microbiol Methods. 2004 Aug;58(2):285-8. doi: 10.1016/j.mimet.2004.04.005.

Authors

Michael J B Lynch¹, Carlos G Leon-Velarde, Scott McEwen, Joseph A Odumeru

Affiliation

¹ Laboratory Services Division, University of Guelph, 95 Stone Road West, Guelph, Ontario, Canada.

PMID: 15234527
DOI: 10.1016/j.mimet.2004.04.005

Abstract

Automated immunomagnetic separation (AIMS), using Dynabeads anti-Salmonella (Dynal, Oslo), was evaluated for its ability to detect Salmonella spp. in poultry environmental samples in comparison with standard, culture-based method (Health Canada, Health Protection Branch, MFHPB-20). AIMS was found to be more reliable in detecting Salmonella from artificially inoculated enrichment broths at low levels and exhibited a 15.5% higher sensitivity value than the culture method.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques* / statistics & numerical data
Food Microbiology*
Humans
Immunomagnetic Separation / methods*
Immunomagnetic Separation / statistics & numerical data
Poultry / microbiology*
Salmonella / isolation & purification*
Salmonella / pathogenicity
Salmonella Food Poisoning / prevention & control
Sensitivity and Specificity