SNPbox: web-based high-throughput primer design from gene to genome

Stefan Weckx; Peter De Rijk; Christine Van Broeckhoven; Jurgen Del-Favero

doi:10.1093/nar/gkh369

SNPbox: web-based high-throughput primer design from gene to genome

Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W170-2. doi: 10.1093/nar/gkh369.

Authors

Stefan Weckx¹, Peter De Rijk, Christine Van Broeckhoven, Jurgen Del-Favero

Affiliation

¹ Department of Molecular Genetics, Bioinformatics Unit, Flanders Interuniversity Institute for Biotechnology, University of Antwerp, Belgium.

Abstract

SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.

MeSH terms

DNA Primers*
Databases, Nucleic Acid
Exons
Genome
Internet
Polymerase Chain Reaction
Polymorphism, Single Nucleotide*
Software*
User-Computer Interface

Substances

DNA Primers