Isolation of a new member of group 3 late embryogenesis abundant protein gene from a halotolerant green alga by a functional expression screening with cyanobacterial cells

Satoshi Tanaka; Kazunori Ikeda; Hitoshi Miyasaka

doi:10.1016/j.femsle.2004.05.019

Isolation of a new member of group 3 late embryogenesis abundant protein gene from a halotolerant green alga by a functional expression screening with cyanobacterial cells

FEMS Microbiol Lett. 2004 Jul 1;236(1):41-5. doi: 10.1016/j.femsle.2004.05.019.

Authors

Satoshi Tanaka¹, Kazunori Ikeda, Hitoshi Miyasaka

Affiliation

¹ The Kansai Electric Power Co., Environmental Research Center, Keihanna-Plaza, 1-7 Seikacho, Sourakugun, Kyoto 619-0237, Japan.

PMID: 15212788
DOI: 10.1016/j.femsle.2004.05.019

Abstract

By a functional expression screening with cyanobacterial cells, a new member of group 3 late embryogenesis abundant protein genes (designated cw80lea3) was isolated from the cDNA library of halotolerant green alga Chlamydomonas sp. strain W80. The principle of the screening method was based on the acquisition of NaCl salt-tolerance of the fresh water cyanobacterial cells carrying the halotolerant algal gene. The expression of cw80lea3 gene in the C. W80 cells was induced by salt- and cold-stresses, but not by abscisic acid treatment.

MeSH terms

Amino Acid Sequence
Animals
Chlamydomonas / genetics*
Chlamydomonas / growth & development*
Chlamydomonas / metabolism
Gene Expression Regulation, Developmental
Molecular Sequence Data
Oxidative Stress / genetics*
Plant Proteins / genetics*
Salts / metabolism
Temperature

Substances

Plant Proteins
Salts
late embryogenesis abundant protein, plant