In order to study seminal roots morphology in barley grown under different water treatments, experiments were carried out under glasshouse-controlled conditions. Eight genotypes were cultivated under four water treatments (100, 75, 50 and 25% of field capacity). Seminal root length and root-to-shoot dry matters' ratio were measured. Root volume was assessed at three soil depths. Results showed broad genotypic differences for all traits. The effect of low and moderate water deficit was slight. In contrast, the impact of severe water treatment was strongly marked on all traits. The impact of water deficit intensity on root traits at different soil depths is discussed.