Fifty (50) strains of Pseudomonas genus have been isolated from the samples of soil, moss and bottom sediments taken on the Galindes, Piterman and Deception Islands (Ukrainian Antarctic station Akademik Vernadsky). Characteristic of the above strains by 84 phenotype features and identification by means of the computer program permitted them to be referred to the species Pseudomonas fluorescens (biovar V.), P. putida (biovars A and B), P. veronii. The belonging of the strain 23 to the species P. veronii has been confirmed by the results of sequence of 16S rRNA. Being grown at low temperatures (4 degrees C) the strains of the above species are characterized by high antagonistic activity as to Gram-positive, Gram-negative bacteria, yeast and microscopic fungi. Cultivation of antagonists at 26 degrees C led to the sharp decrease or complete disappearance of the antagonistic effect.