Induction of partial protection in mice after oral administration of Lactococcus lactis producing Brucella abortus L7/L12 antigen

J Drug Target. 2003;11(8-10):489-93. doi: 10.1080/10611860410001670035.

Abstract

The Brucella abortus ribosomal protein L7/L12 is an immunodominant antigen and an interesting candidate for the development of oral live vaccines against brucellosis. Here, a recombinant Lactococcus lactis strain producing L7/L12 under the control of nisin inducible promoter was orally administered to BALB/c mice. Significant levels of anti-L7/L12 specific IgA detected in feces revealed an induced local humoral immune response. However, serum analysis did not reveal any anti-L7/L12 antibodies suggesting the absence of a systemic response. Nevertheless, the vaccinated mice showed a partial protective immunity against B. abortus virulent strain (S2308) challenged by intraperitoneal inoculation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Brucella Vaccine / administration & dosage*
  • Brucella Vaccine / genetics
  • Brucella Vaccine / immunology*
  • Brucella abortus / genetics
  • Brucella abortus / immunology*
  • Brucellosis / immunology
  • Brucellosis / prevention & control
  • Enzyme-Linked Immunosorbent Assay
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / immunology*

Substances

  • Antigens, Bacterial
  • Brucella Vaccine
  • Ribosomal Proteins
  • ribosomal protein L7-L12