Amphetamines and 3,4-methylendioxymetamphetamine (MDMA): evaluation of KIMS (kinetic interaction of microparticles in solution) assay at two cut-off levels

Clin Chem Lab Med. 2004 May;42(5):543-8. doi: 10.1515/CCLM.2004.092.

Abstract

Two screening methods for the assay of amphetamines and their derivatives have been applied to the same analytical instrument for their evaluation. In addition to an assay at a cut-off of 1000 microg/l, a new specific reagent was evaluated for an ultra-sensitive assay of amphetamines and 3,4-methylendioxymetamphetamine with a cut-off of 300 microg/l. The assay confirmation was performed using high-performance liquid chromatography and gas chromatography/ mass spectrometry techniques. The results were positive for both screening methods, confirming the efficacy of two simultaneous methods with different cut-off levels.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • 3,4-Methylenedioxyamphetamine / urine
  • Adolescent
  • Adult
  • Amphetamines / urine*
  • Chromatography, High Pressure Liquid
  • Clinical Chemistry Tests / methods*
  • Clinical Chemistry Tests / statistics & numerical data
  • Enzyme Multiplied Immunoassay Technique / statistics & numerical data
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Immunoassay / methods*
  • Immunoassay / statistics & numerical data
  • Male
  • Methamphetamine / urine
  • Middle Aged
  • N-Methyl-3,4-methylenedioxyamphetamine / urine*
  • Nanostructures
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Substance-Related Disorders / diagnosis
  • Substance-Related Disorders / urine

Substances

  • Amphetamines
  • Methamphetamine
  • 3,4-Methylenedioxyamphetamine
  • N-Methyl-3,4-methylenedioxyamphetamine