Using static dark enclosed chamber technique, the emissions of CO2 from typical temperate grasslands such as Stipa baicalensis, Leymus chinensis, Stipa grandis and Stipa krylovii in Xilin River Basin of Inner Mongolia were measured. And the contents of organic carbon and total nitrogen in different soil layers were also studied to analyze the correlation between the emissions of CO2 and the contents of C & N. The results showed that the emissions of CO2 from the 4 grassland communities were significantly positively correlated with soil organic carbon and total nitrogen in different layers of grassland soils, and the correlation coefficients were almost all above 0.8. That meant the flux of CO2 is severely influenced by the contents of soil organic carbon and total nitrogen when environmental factors were similar. The decreases of soil organic carbon and total nitrogen with the depth into soils (more than 70% of the contents centralizes above 0-30 cm layer) showed that most of them came from the decomposition of organic matter on the earth's surface. In the 0-100 cm layer of grassland soils, the decreases of amount of soil organic carbon and total nitrogen from Stipa baicalensis, Stipa grandis and Leymus chinensis grasslands to Stipa krylovii grassland orderly showed that the impacts of precipitation, evaporation and dryness index on the distribution of soil organic carbon and total nitrogen were important.