[Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2004 May;33(3):189-92. doi: 10.3785/j.issn.1008-9292.2004.03.002.
[Article in Chinese]

Abstract

Objective: To determine the effect of histamine on N-methyl-D-aspartate (NMDA) induced neuron death and to elucidate its mechanism.

Methods: The primary cortical cell culture was adopted. Neuron morphology and MTT assay were used to evaluate the drugs effects.

Result: Histamine at doses of 10(-4) 10(-6) 10(-7) 10(-8) mol/L reversed the neuron death induced by NMDA (50 micromol/L) for 3 h. The protection of histamine peaked at doses of 10(-4) mol/L and 10(-7)mol/L. The effect of histamine of 10(-7) mol/L was reversed only by cimetidine an H(2)receptor antagonist. However, the effect of histamine of 10(-4) mol/L was reversed only by pyrilamine but not cimetidine.

Conclusion: Histamine could reduce neuron death induced by NMDA; its protection at a low dose might be mediated by H(2)receptor, and at a high dose by H(1)receptor.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Histamine / pharmacology*
  • N-Methylaspartate / toxicity*
  • Neurons / drug effects*
  • Neuroprotective Agents / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Histamine H1 / physiology
  • Receptors, Histamine H2 / physiology

Substances

  • Neuroprotective Agents
  • Receptors, Histamine H1
  • Receptors, Histamine H2
  • N-Methylaspartate
  • Histamine