Isolation of high quality RNA from Streptomyces

J Microbiol Methods. 2004 Jul;58(1):135-7. doi: 10.1016/j.mimet.2004.03.015.

Abstract

Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Edetic Acid / chemistry
  • Guanidines / chemistry
  • Octoxynol / chemistry
  • Phenols / chemistry
  • RNA, Bacterial / isolation & purification*
  • Streptomyces / chemistry
  • Streptomyces / genetics*

Substances

  • Guanidines
  • Phenols
  • RNA, Bacterial
  • trizol
  • Octoxynol
  • Edetic Acid