Development of a PCR assay for Streptococcus iniae based on the lactate oxidase (lctO) gene with potential diagnostic value

Vet Microbiol. 2004 Jun 21;101(2):109-16. doi: 10.1016/j.vetmic.2004.03.012.

Abstract

Streptococcus iniae is a well-known pathogen of both fish and humans that is difficult to identify by conventional biochemical tests. A PCR assay based on the lactate oxidase (lctO) gene of S. iniae was developed for the rapid and specific detection and identification of this pathogen from different sources. The PCR assay had a detection limit of 62-31 cells, and 25 pg of DNA per PCR reaction mixture. The PCR was also effective in detecting the bacterium from inoculated tissue homogenates, suggesting its potential use for a rapid and accurate diagnosis of S. iniae infections.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aquaculture
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Fish Diseases / diagnosis
  • Fish Diseases / microbiology*
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / genetics*
  • Oncorhynchus mykiss*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Sea Bream*
  • Sensitivity and Specificity
  • Streptococcal Infections / diagnosis
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / veterinary*
  • Streptococcus / enzymology
  • Streptococcus / genetics*

Substances

  • DNA, Bacterial
  • Mixed Function Oxygenases
  • lactate 2-monooxygenase