Abstract
3-Hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase is the rate-limiting enzyme in the biosynthesis of cholesterol in mammals. Some microbial metabolites have been found to be HMG-CoA reductase inhibitors. Korean soybean paste is a unique food fermented by many microorganisms. The enzymatic method using the catalytic domain of Syrian hamster HMG-CoA reductase was employed for the screening of HMG-CoA reductase inhibitors. Soybean paste extract was fractionated by vacuum liquid chromatography. Fractions showing relatively high HMG-CoA reductase inhibition were further purified through Sephadex LH-20 column chromatography and C18 preparative HPLC, and the inhibitory compounds were identified as genistein, daidzein, and glycitein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatography, High Pressure Liquid
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Cricetinae
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Genistein / analysis
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Glycine max / chemistry*
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Hydroxymethylglutaryl-CoA Reductase Inhibitors / chemistry*
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Hydroxymethylglutaryl-CoA Reductase Inhibitors / isolation & purification*
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Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology
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Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / metabolism
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Isoflavones / analysis
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Isoflavones / chemistry
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Isoflavones / isolation & purification*
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Isoflavones / pharmacology*
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Mass Spectrometry
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Molecular Structure
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Plant Extracts / chemistry*
Substances
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Hydroxymethylglutaryl-CoA Reductase Inhibitors
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Isoflavones
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Plant Extracts
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daidzein
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glycitein
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Genistein
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Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent