Qualitative method for determination of aflatoxin B1 in nuts

J AOAC Int. 2004 Mar-Apr;87(2):417-23.

Abstract

The proper characterization of a commercial qualitative method for determining aflatoxin B1 in some nuts is described. A qualitative method that provides binary responses of the yes/no type means that the performance parameters have been properly adapted and defined. Performance characteristics such as the cut-off limit, the detection limit, sensitivity, specificity, false-positive and negative rates, and the unreliability or uncertainty region are defined and then estimated by means of the performance characteristic curves. The commercial test kit showed the cut-off limit at 1.6 ng/g, with a sensitivity rate of 95% and a false-negative rate of zero. A modification can be performed to shift the cut-off to 2.0 ng/g, keeping the same values for the sensitivity and false-negative rate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin B1 / analysis*
  • Nuts / chemistry*
  • Reagent Kits, Diagnostic

Substances

  • Reagent Kits, Diagnostic
  • Aflatoxin B1