The fate of mitochondria in Ibex-hirus reconstructed early embryos

Acta Biochim Biophys Sin (Shanghai). 2004 May;36(5):371-4. doi: 10.1093/abbs/36.5.371.

Abstract

Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified / embryology
  • Cloning, Organism / methods*
  • DNA, Mitochondrial / genetics
  • Embryo, Mammalian / physiology*
  • Embryo, Mammalian / surgery*
  • Embryonic and Fetal Development / genetics
  • Mitochondria / physiology*
  • Nuclear Transfer Techniques*
  • Ruminants / embryology*
  • Ruminants / genetics*

Substances

  • DNA, Mitochondrial