Serial bull cloning by somatic cell nuclear transfer

Nat Biotechnol. 2004 Jun;22(6):693-4. doi: 10.1038/nbt975. Epub 2004 May 23.

Abstract

Although the list of species successfully cloned continues to grow, serial cloning has not been reported in species other than the mouse. Here we describe two live births of second-generation clones of a bull. Clones of the first and second generations appear healthy and have normal telomere lengths. Our attempts to produce the third generation of clones were unsuccessful.

MeSH terms

  • Abortion, Veterinary
  • Animals
  • Blastomeres / cytology
  • Cattle
  • Cell Count
  • Cleavage Stage, Ovum / cytology
  • Cloning, Organism / methods*
  • Embryo Transfer / veterinary
  • Female
  • Fertility / physiology
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Nuclear Transfer Techniques*
  • Oocytes / cytology
  • Pregnancy
  • Pregnancy Outcome
  • Pregnancy Rate
  • Telomerase / genetics
  • Telomerase / metabolism
  • Telomere / genetics
  • Telomere / metabolism

Substances

  • Telomerase