Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis

Emil P Kartalov; Stephen R Quake

doi:10.1093/nar/gkh613

Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis

Nucleic Acids Res. 2004 May 20;32(9):2873-9. doi: 10.1093/nar/gkh613. Print 2004.

Authors

Emil P Kartalov¹, Stephen R Quake

Affiliation

¹ Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.

Publication types

Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Pairing
Base Sequence
DNA / biosynthesis
DNA / chemistry
Microfluidics / instrumentation*
Sequence Analysis, DNA / instrumentation*
Sequence Analysis, DNA / methods

Substances

DNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding