Abstract
A Salmonella enterica serovar Typhimurium aroA-deficient delivery system was used to target the immunosuppressive protein Salp15 to antigen-presenting cells. In vitro and in vivo infections with Salp15-containing Salmonella resulted in an impaired CD4(+)-T-cell activation, suggesting that the protein was produced by antigen-presenting cells in a physiologically active form.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3-Phosphoshikimate 1-Carboxyvinyltransferase
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Alkyl and Aryl Transferases / genetics
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Animals
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / metabolism*
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Antigen-Presenting Cells / microbiology
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / pathology
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Cells, Cultured
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Genetic Vectors*
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Lymphocyte Activation / physiology
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Macrophages, Alveolar
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Mice
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Mice, Inbred BALB C
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Mutation
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Salivary Proteins and Peptides / genetics
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Salivary Proteins and Peptides / metabolism*
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Salmonella typhimurium / genetics
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Salmonella typhimurium / pathogenicity*
Substances
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Salivary Proteins and Peptides
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Salp15 protein, Ixodes scapularis
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Alkyl and Aryl Transferases
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3-Phosphoshikimate 1-Carboxyvinyltransferase