Delivery of the immunosuppressive antigen Salp15 to antigen-presenting cells by Salmonella enterica serovar Typhimurium aroA mutants

Infect Immun. 2004 Jun;72(6):3638-42. doi: 10.1128/IAI.72.6.3638-3642.2004.

Abstract

A Salmonella enterica serovar Typhimurium aroA-deficient delivery system was used to target the immunosuppressive protein Salp15 to antigen-presenting cells. In vitro and in vivo infections with Salp15-containing Salmonella resulted in an impaired CD4(+)-T-cell activation, suggesting that the protein was produced by antigen-presenting cells in a physiologically active form.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Phosphoshikimate 1-Carboxyvinyltransferase
  • Alkyl and Aryl Transferases / genetics
  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism*
  • Antigen-Presenting Cells / microbiology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / pathology
  • Cells, Cultured
  • Genetic Vectors*
  • Lymphocyte Activation / physiology
  • Macrophages, Alveolar
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Salivary Proteins and Peptides / genetics
  • Salivary Proteins and Peptides / metabolism*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / pathogenicity*

Substances

  • Salivary Proteins and Peptides
  • Salp15 protein, Ixodes scapularis
  • Alkyl and Aryl Transferases
  • 3-Phosphoshikimate 1-Carboxyvinyltransferase