Abstract
Increasing evidence suggests a major role for phosphatidylcholine (PC) in plant stress adaptation. The present work investigated the regulation of choline, PC and interconnected phosphatidylethanolamine biosynthesis in Arabidopsis thaliana L. as a function of cold- and salt- or mannitol-mediated hyperosmotic stresses. While PC synthesis is accelerated in both salt- and cold-treated plants, the choline kinase (CK) and phosphocholine cytidylyltransferase genes are oppositely regulated with respect to these abiotic treatments. Salt stress also stimulates CK activity in vitro. A possible regulatory role of CK in stimulating PC biosynthesis rate in salt-stressed plants is discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Arabidopsis / enzymology*
-
Arabidopsis / metabolism
-
Blotting, Northern
-
Carbon Radioisotopes
-
Choline Kinase / metabolism*
-
Cold Temperature
-
Cytidine Diphosphate Choline / metabolism
-
Cytosol / enzymology
-
Gene Expression
-
Hypertonic Solutions / pharmacology
-
Mannitol / pharmacology
-
Osmotic Pressure
-
Phosphatidylcholines / biosynthesis*
-
Phosphatidylcholines / genetics
-
Phosphatidylethanolamines / biosynthesis
-
Sodium Chloride / pharmacology*
-
Transcription, Genetic / drug effects
Substances
-
Carbon Radioisotopes
-
Hypertonic Solutions
-
Phosphatidylcholines
-
Phosphatidylethanolamines
-
phosphatidylethanolamine
-
Mannitol
-
Sodium Chloride
-
Cytidine Diphosphate Choline
-
Choline Kinase