Soil microbial features in Lipu copper mining and non-mine soil were studied comparatively. The results indicated that mine soil possessed obviously different microbial features such as lower microbial biomass carbon and soil basal respiration strength, Cmic/Corg decreasing, and higher microbial ecophysiological parameters qCO2, indicating that heavy metal had a depressive impact on soil microbial eco-characteristics. Biolog data showed that mine soil microbial community structure was changed obviously, the speed and quantity of carbon consuming were increased significantly, and the kinds of carbon sources which soil microorganism used were changed, led to consume much more energies for maintaining the normal needs of its life. But the utilization efficiency was lower compared with the control. All the results showed that soil microbial eco-characteristics could be used as a sensitive, effective and liable index of mine soil environment qualities.