Abstract
Resveratrol, a polyphenol compound found in grapes and red wines, is a prominent anti-cancer agent. In this study, we demonstrate that resveratrol enhanced TNF-alpha, IL-12 and IL-1beta production from LPS activated phorbol myristate acetate (PMA) differentiated THP-1 human macrophages. Expression of CD86 on macrophages was enhanced by resveratrol alone and with LPS. When macrophages were primed with IFN-gamma, resveratrol suppressed the expression of HLA-ABC, HLA-DR, CD80, CD86 and inhibited production of TNF-alpha, IL-12, IL-6 and IL-1beta induced by LPS. The differential impact of resveratrol on expression of CD14 might be correlated with differential response of macrophages to LPS with or without IFN-gamma priming.
Copyright 2004 Elsevier B.V.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antigen-Presenting Cells / drug effects
-
Antigens, CD / biosynthesis
-
B7-2 Antigen
-
Cell Death / drug effects
-
Cell Differentiation / drug effects
-
Cell Survival / drug effects
-
Cytokines / biosynthesis
-
Enzyme-Linked Immunosorbent Assay
-
Flow Cytometry
-
Humans
-
In Vitro Techniques
-
Interferon-gamma / pharmacology*
-
Interleukin-1 / biosynthesis
-
Interleukin-12 / biosynthesis
-
Lipopolysaccharide Receptors / biosynthesis
-
Lipopolysaccharides / pharmacology*
-
Macrophages / drug effects*
-
Membrane Glycoproteins / biosynthesis
-
Phenotype
-
Recombinant Proteins
-
Resveratrol
-
Stilbenes / pharmacology*
-
Tetradecanoylphorbol Acetate / pharmacology
-
Tetrazolium Salts
-
Thiazoles
-
Thymidine / metabolism
-
Tumor Necrosis Factor-alpha / biosynthesis
Substances
-
Antigens, CD
-
B7-2 Antigen
-
CD86 protein, human
-
Cytokines
-
Interleukin-1
-
Lipopolysaccharide Receptors
-
Lipopolysaccharides
-
Membrane Glycoproteins
-
Recombinant Proteins
-
Stilbenes
-
Tetrazolium Salts
-
Thiazoles
-
Tumor Necrosis Factor-alpha
-
Interleukin-12
-
Interferon-gamma
-
thiazolyl blue
-
Tetradecanoylphorbol Acetate
-
Resveratrol
-
Thymidine