Cultured neurons coupled to microelectrode arrays: circuit models, simulations and experimental data

Sergio Martinoia; Paolo Massobrio; Marco Bove; Giuseppe Massobrio

doi:10.1109/TBME.2004.826607

Cultured neurons coupled to microelectrode arrays: circuit models, simulations and experimental data

IEEE Trans Biomed Eng. 2004 May;51(5):859-64. doi: 10.1109/TBME.2004.826607.

Authors

Sergio Martinoia¹, Paolo Massobrio, Marco Bove, Giuseppe Massobrio

Affiliation

¹ Neuroengineering and Bio-nanoTechnology Group, Department of Biophysical and Electronic Engineering, University of Genova, Via Opera Pia 11a, 16145 Genova, Italy. giaser@dibe.unige.it

PMID: 15132514
DOI: 10.1109/TBME.2004.826607

Abstract

The purpose of this paper is to characterize the neuron-microelectrode junction, based on the equivalent electric-circuit approach. As a result, recording of action potentials can be simulated with a general-purpose circuit simulation program such as HSPICE. The response of the microelectrode was analyzed as a function of parameters such as sealing resistance and adhesion conditions. The models of the neuron and microelectrode implemented in HSPICE were first described. These models were used to simulate the behavior of the junction between a patch of neuronal membrane (described by the compartmental model) and a microelectrode.

Publication types

Comparative Study
Evaluation Study
Validation Study

MeSH terms

Action Potentials / physiology*
Animals
Cell Culture Techniques / methods*
Cells, Cultured
Computer Simulation
Electrophysiology / methods*
Equipment Failure Analysis / methods*
Microelectrodes*
Models, Neurological*
Neurons / physiology*
Rats