Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species

Caroline Massonet; Johan Van Eldere; Mario Vaneechoutte; Thierry De Baere; Jan Verhaegen; Katrien Lagrou

doi:10.1128/JCM.42.5.2209-2211.2004

Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species

J Clin Microbiol. 2004 May;42(5):2209-11. doi: 10.1128/JCM.42.5.2209-2211.2004.

Authors

Caroline Massonet¹, Johan Van Eldere, Mario Vaneechoutte, Thierry De Baere, Jan Verhaegen, Katrien Lagrou

Affiliation

¹ Department of Immunology and Microbiology, Rega Institute, U.Z. Gasthuisberg, B-3000 Leuven, Belgium. caroline.massonet@med.kuleuven.ac.be

Abstract

A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Candida / classification
Candida / genetics
Candida / isolation & purification
Cryptococcus / classification
Cryptococcus / genetics
Cryptococcus / isolation & purification
DNA, Fungal / genetics
DNA, Fungal / isolation & purification
Humans
Mycology / methods*
Mycoses / diagnosis
Mycoses / microbiology
Polymerase Chain Reaction
Polymorphism, Genetic
Saccharomyces / classification
Saccharomyces / genetics
Saccharomyces / isolation & purification
Saccharomycetales / classification
Saccharomycetales / genetics
Saccharomycetales / isolation & purification
Yeasts / classification*
Yeasts / genetics*
Yeasts / isolation & purification

Substances

DNA, Fungal