Detection of HIV-1 subtype G using Cobas Ampliscreen test

Fernando M Araújo; Isabel S Henriques; Fátima P Monteiro; Elsa R Meireles; Luis M Cunha-Ribeiro

doi:10.1016/j.jcv.2003.11.001

Detection of HIV-1 subtype G using Cobas Ampliscreen test

J Clin Virol. 2004 Jun;30(2):205-6. doi: 10.1016/j.jcv.2003.11.001.

Authors

Fernando M Araújo¹, Isabel S Henriques, Fátima P Monteiro, Elsa R Meireles, Luis M Cunha-Ribeiro

Affiliation

¹ Department of Transfusion Medicine and Blood Bank, Molecular Biology Center, Serv. Imuno-hemoterapia/Hospital S. João, Hospital S. João, 4200 Porto, Portugal. araujo@hsjoao.min-saude.pt

PMID: 15125878
DOI: 10.1016/j.jcv.2003.11.001

Abstract

A nucleic acid amplification test for detection of HIV-1 RNA was designed for screening pools of human plasma. This test achieves a similar level of sensitivity for group M subtypes, but few samples of subtype G and none of its CRFs had been tested, which are the most relevant in Portugal. We found that the test is effective in detecting HIV-1 subtypes and has an analytical sensitivity similar to B subtype.

MeSH terms

Acquired Immunodeficiency Syndrome / diagnosis
Acquired Immunodeficiency Syndrome / epidemiology
HIV-1 / classification*
HIV-1 / isolation & purification
Humans
RNA, Viral / analysis*
Reproducibility of Results
Sensitivity and Specificity

Substances

RNA, Viral