From the hexane extract of the underground parts of Eleocharis acutangula (Roxb.) Schult., lup-20(29)-ene-3beta,16beta-diol and a mixture of campesterol, stigmasterol and sitosterol were isolated. The hexane extracts of aerial and underground parts of E. sellowiana Kunth furnished two new substances, namely neohop-13(18)-en-3alpha-ol and stigmast-22-en-3beta,6beta,9alpha-triol, together with a mixture of steroids, betulinic acid, stigmast-4-en-6beta-ol-3-one and fern-9(11)-en-3alpha-ol. The molecular structures were determined by spectral analysis (1D- and 2D-NMR experiments and MS) and comparison with literature data.