The KlSRB10 gene from Kluyveromyces lactis

Yeast. 2004 Apr 30;21(6):511-8. doi: 10.1002/yea.1117.

Abstract

We report the cloning and sequencing of a gene from Kluyveromyces lactis with high homology to the SRB10 gene (alias UME5, SSN3, GIG2, NUT7, RYE5) from Saccharomyces cerevisiae and other organisms. The KlSRB10 gene is located in a similar configuration to that found in S. cerevisiae, flanked by NOT4 and a gene with high similarity to YPL041c. The translated protein contains 593 amino acids and the characteristic domains of kinases from the CMGC subgroup. The functional relationship to yeast SRB10 is demonstrated by complementation of mutant phenotypes in a haploid S. cerevisiae strain containing a null allele.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases / genetics*
  • Cyclin-Dependent Kinases / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Genetic Complementation Test
  • Kluyveromyces / genetics*
  • Kluyveromyces / metabolism
  • Molecular Sequence Data
  • RNA, Fungal / chemistry
  • RNA, Fungal / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Fungal Proteins
  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases
  • SSN3 protein, S cerevisiae

Associated data

  • GENBANK/AJ532841