Structure of a highly phosphorylated O-polysaccharide of Proteus mirabilis O41

Sof'ya N Senchenkova; Andrei V Perepelov; Maciej Cedzynski; Anna S Swierzko; Andrzej Ziolkowski; Alexander S Shashkov; Wieslaw Kaca; Yuriy A Knirel; Per-Erik Jansson

doi:10.1016/j.carres.2004.02.006

Structure of a highly phosphorylated O-polysaccharide of Proteus mirabilis O41

Carbohydr Res. 2004 May 17;339(7):1347-52. doi: 10.1016/j.carres.2004.02.006.

Authors

Sof'ya N Senchenkova¹, Andrei V Perepelov, Maciej Cedzynski, Anna S Swierzko, Andrzej Ziolkowski, Alexander S Shashkov, Wieslaw Kaca, Yuriy A Knirel, Per-Erik Jansson

Affiliation

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.

PMID: 15113673
DOI: 10.1016/j.carres.2004.02.006

Abstract

A highly phosphorylated O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O41 followed by GPC. The initial and dephosphorylated polysaccharides and phosphorylated products from two sequential Smith degradations were studied by (1)H, (13)C and (31)P NMR spectroscopy and ESI-MS. The O-polysaccharide was found to have a tetrasaccharide repeating unit containing one ribitol phosphate (presumably d-Rib-ol-5-P) and two ethanolamine phosphate (Etn-P) groups, one of which is present in the stoichiometric amount and the other in a nonstoichiometric amount. The following structure of the O-polysaccharide was established:

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
O Antigens / chemistry*
O Antigens / isolation & purification
O Antigens / metabolism
Pentosephosphates / chemistry
Phosphorylation
Proteus mirabilis / chemistry*

Substances

O Antigens
Pentosephosphates
ribitol-5-phosphate