Abstract
In biochip development two issues are critical: stable and specific immobilization of the ligand and achievement of high signal-to-background ratio. In this work we have addressed these issues for the development of biochips, produced by sputtering multilayers of thin metal films, metal oxides, and metal nitrides (tens to hundreds of nanometers thick) onto glass wafers. Optimized surfaces have shown good results in genomic and proteomic experiments with biochips based on surface-enhanced fluorescence and absorption techniques.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Adsorption
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Coated Materials, Biocompatible / chemical synthesis
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Coated Materials, Biocompatible / chemistry*
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Crystallization / methods*
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Equipment Design
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Equipment Failure Analysis
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Gases
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Hot Temperature
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Materials Testing
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Membranes, Artificial
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Metals / chemistry*
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Nanotechnology / instrumentation*
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Nanotechnology / methods
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Nanotubes / chemistry*
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Nanotubes / ultrastructure*
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Oligonucleotide Array Sequence Analysis / instrumentation*
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Oligonucleotide Array Sequence Analysis / methods
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Protein Array Analysis / instrumentation*
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Protein Array Analysis / methods
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Reproducibility of Results
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Sensitivity and Specificity
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Surface Properties
Substances
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Coated Materials, Biocompatible
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Gases
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Membranes, Artificial
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Metals