Tuning the setup of sputter-coated multilayers in nanocluster-based signal-enhancing biochips for optimal performance in protein and DNA assays

J Nanosci Nanotechnol. 2004 Jan-Feb;4(1-2):98-105. doi: 10.1166/jnn.2004.228.

Abstract

In biochip development two issues are critical: stable and specific immobilization of the ligand and achievement of high signal-to-background ratio. In this work we have addressed these issues for the development of biochips, produced by sputtering multilayers of thin metal films, metal oxides, and metal nitrides (tens to hundreds of nanometers thick) onto glass wafers. Optimized surfaces have shown good results in genomic and proteomic experiments with biochips based on surface-enhanced fluorescence and absorption techniques.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adsorption
  • Coated Materials, Biocompatible / chemical synthesis
  • Coated Materials, Biocompatible / chemistry*
  • Crystallization / methods*
  • Equipment Design
  • Equipment Failure Analysis
  • Gases
  • Hot Temperature
  • Materials Testing
  • Membranes, Artificial
  • Metals / chemistry*
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods
  • Nanotubes / chemistry*
  • Nanotubes / ultrastructure*
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Surface Properties

Substances

  • Coated Materials, Biocompatible
  • Gases
  • Membranes, Artificial
  • Metals