Recent developments in the optimization of thermostable DNA polymerases for efficient applications

Trends Biotechnol. 2004 May;22(5):253-60. doi: 10.1016/j.tibtech.2004.02.011.

Authors

Andrey R Pavlov¹, Nadejda V Pavlova, Sergei A Kozyavkin, Alexei I Slesarev

Affiliation

¹ Fidelity Systems Inc., 7961 Cessna Avenue, Gaithersburg, Maryland 20879, USA.

PMID: 15109812
DOI: 10.1016/j.tibtech.2004.02.011

No abstract available

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Review

MeSH terms

Archaea / enzymology*
Archaea / genetics
Archaeal Proteins / chemistry*
Archaeal Proteins / genetics
Bacteria / enzymology*
Bacteria / genetics
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
DNA Polymerase I / chemistry*
DNA Polymerase I / genetics
DNA Primers
DNA-Directed RNA Polymerases / chemistry
Deoxyribonucleotides / chemistry
Enzyme Stability / genetics
Hot Temperature
Kinetics
Polymerase Chain Reaction / methods
Sequence Analysis, DNA / methods
Viral Proteins

Substances

Archaeal Proteins
Bacterial Proteins
DNA Primers
Deoxyribonucleotides
Viral Proteins
bacteriophage T7 RNA polymerase
DNA-Directed RNA Polymerases
DNA Polymerase I