A novel, ultra-large xylanolytic complex (xylanosome) secreted by Streptomyces olivaceoviridis

Biotechnol Lett. 2004 Mar;26(5):431-6. doi: 10.1023/b:bile.0000018266.75248.03.

Abstract

The production of a novel, very large xylanolytic complex (xylanosome) by Streptomyces olivaceoviridis E-86 is reported. The molecular weight was approx. 1200 kDa as determined by native gradient gel electrophoresis. Corncob xylan was the best inducer of xylanosome formation. The xylanosome was produced when the organism was grown for 5 d between pH 4.5-6 and at 27.5 to 35 degrees C.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Endo-1,4-beta Xylanases / biosynthesis*
  • Endo-1,4-beta Xylanases / chemistry
  • Enzyme Stability
  • Hydrogen-Ion Concentration
  • Molecular Weight
  • Multienzyme Complexes / biosynthesis*
  • Multienzyme Complexes / chemistry
  • Species Specificity
  • Streptomyces / chemistry
  • Streptomyces / classification
  • Streptomyces / enzymology*
  • Streptomyces / growth & development
  • Temperature
  • Xylans / classification
  • Xylans / metabolism*

Substances

  • Multienzyme Complexes
  • Xylans
  • Endo-1,4-beta Xylanases