A novel Streptococcus organism identified in a case of fulminant endocarditis using 16S rDNA sequencing

Zsolt Jobbagy; Claire B Fabian; Vincent A Memoli; Joseph D Schwartzman

doi:10.1016/S1525-1578(10)60503-X

A novel Streptococcus organism identified in a case of fulminant endocarditis using 16S rDNA sequencing

J Mol Diagn. 2004 May;6(2):145-8. doi: 10.1016/S1525-1578(10)60503-X.

Authors

Zsolt Jobbagy¹, Claire B Fabian, Vincent A Memoli, Joseph D Schwartzman

Affiliation

¹ Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA. zsolt_jobbagy@hitchcock.org

Abstract

We report the identification of a virulent Streptococcus organism associated with fulminant endocarditis, using 16S rRNA gene amplification, sequencing and assembly from formalin-fixed, paraffin-embedded archival heart valve tissue, years after the autopsy of a patient.

Publication types

Case Reports

MeSH terms

Aortic Diseases / etiology
Autopsy
DNA, Bacterial / genetics*
DNA, Ribosomal / analysis*
Endocarditis, Bacterial / diagnosis*
Endocarditis, Bacterial / microbiology
Female
Gene Amplification
Heart Valves
Humans
Middle Aged
Paraffin Embedding
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Streptococcal Infections / diagnosis*
Streptococcal Infections / microbiology
Streptococcus / genetics*

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S