Tight binding ligand approach to oligosaccharide-grafted protein

Bioorg Med Chem Lett. 2004 May 3;14(9):2285-9. doi: 10.1016/j.bmcl.2004.01.106.

Abstract

A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Ligands
  • Methotrexate / chemistry
  • Molecular Sequence Data
  • Oligosaccharides / chemistry
  • Oligosaccharides / metabolism*
  • Protein Binding
  • Tetrahydrofolate Dehydrogenase / chemistry
  • Tetrahydrofolate Dehydrogenase / metabolism*

Substances

  • Ligands
  • Oligosaccharides
  • Tetrahydrofolate Dehydrogenase
  • Methotrexate