Abstract
A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Sequence
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Electrophoresis, Polyacrylamide Gel
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Ligands
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Methotrexate / chemistry
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Molecular Sequence Data
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism*
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Protein Binding
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Tetrahydrofolate Dehydrogenase / chemistry
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Tetrahydrofolate Dehydrogenase / metabolism*
Substances
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Ligands
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Oligosaccharides
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Tetrahydrofolate Dehydrogenase
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Methotrexate