Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector

Jennifer L Bromberg-White; Craig P Webb; Veronique S Patacsil; Cindy K Miranti; Bart O Williams; Sheri L Holmen

doi:10.1128/jvi.78.9.4914-4916.2004

Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector

J Virol. 2004 May;78(9):4914-6. doi: 10.1128/jvi.78.9.4914-4916.2004.

Authors

Jennifer L Bromberg-White¹, Craig P Webb, Veronique S Patacsil, Cindy K Miranti, Bart O Williams, Sheri L Holmen

Affiliation

¹ Laboratory of Tumor Metastasis and Angiogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.

Abstract

While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birds / virology*
Cell Line
Cell Line, Tumor
Fibroblasts
Genetic Vectors*
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
Humans
RNA, Small Interfering / genetics*
Retroviridae / genetics*
Retroviridae / physiology
Virus Replication*

Substances

RNA, Small Interfering
Glyceraldehyde-3-Phosphate Dehydrogenases