Alpha-amylase inhibitors from Ficus sp. seeds and their activities towards coleoptera insect pests

Protein Pept Lett. 2004 Apr;11(2):181-7. doi: 10.2174/0929866043478329.

Abstract

Alpha-amylase Inhibitors were isolated from Ficus sp. (Gameleira) seeds by acetone fractionation and Sephadex G-50. Two inhibitors (alpha-PPAI and alpha-ZSAI) were tested against alpha-amylases from coleopteran larvae. alpha-PPAI was active to alpha-amylases of Callosobruchus maculatus (52%) and Zabrotes subfasciatus (53%). alpha-ZSAI was strongly active to Z. subfasciatus (100%) of and Mimosestes mimosae (98%). The alpha-ZSAI is a glycoprotein of approximately 50 kDa with an IC50 value of 0.074 microg microl(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coleoptera / drug effects*
  • Coleoptera / enzymology
  • Ficus / chemistry*
  • Larva / drug effects
  • Larva / enzymology
  • Pest Control*
  • Plant Proteins / chemistry*
  • Plant Proteins / toxicity
  • Seeds / chemistry
  • Trypsin Inhibitors
  • alpha-Amylases / antagonists & inhibitors

Substances

  • Plant Proteins
  • Trypsin Inhibitors
  • alpha-Amylases