Evaluation of different methods to determine the loading of proteins in PLGA microspheres

C H Zheng; W Q Liang; H Y Yu; H L Chen

Evaluation of different methods to determine the loading of proteins in PLGA microspheres

Pharmazie. 2004 Mar;59(3):232-3.

Authors

C H Zheng¹, W Q Liang, H Y Yu, H L Chen

Affiliation

¹ Department of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR China.

PMID: 15074601

Abstract

Various determination methods for protein encapsulation efficiency of PLGA microspheres were compared. Acetonitrile is recommended as an extraction solvent.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetonitriles
Drug Compounding / methods*
Lactic Acid
Methylene Chloride
Microspheres
Particle Size
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Polymers
Proteins / analysis*
Proteins / chemistry
Serum Albumin, Bovine / chemistry
Solubility
Solvents

Substances

Acetonitriles
Polymers
Proteins
Solvents
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Serum Albumin, Bovine
Lactic Acid
Methylene Chloride
acetonitrile