Reactivation of porcine cytomegalovirus through allogeneic stimulation

J Clin Microbiol. 2004 Apr;42(4):1756-8. doi: 10.1128/JCM.42.4.1756-1758.2004.

Abstract

Reactivation of latent porcine cytomegalovirus after coculture of peripheral blood mononuclear cells (PBMCs) from pigs with different genetic backgrounds was investigated. Nine of 10 allogeneic coculture pairs were PCR (DNA) positive, whereas 7 coculture pairs had porcine cytomegalovirus (PCMV) RNA, an indication of virus replication. The cell subpopulations harboring PCMV were monocytes and CD8+ T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Coculture Techniques
  • Cytomegalovirus / physiology*
  • Cytomegalovirus Infections / virology
  • DNA, Viral / analysis
  • Leukocytes, Mononuclear / physiology
  • Leukocytes, Mononuclear / virology*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine / virology*
  • Transplantation, Homologous*
  • Virus Activation*
  • Virus Latency

Substances

  • DNA, Viral
  • RNA, Messenger
  • RNA, Viral