NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters

Umberto Tomati; Monica Belardinelli; Emanuela Galli; Valentina Iori; Donatella Capitani; Luisa Mannina; Stéphane Viel; Annalaura Segre

doi:10.1016/j.carres.2004.02.007

NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters

Carbohydr Res. 2004 Apr 28;339(6):1129-34. doi: 10.1016/j.carres.2004.02.007.

Authors

Umberto Tomati¹, Monica Belardinelli, Emanuela Galli, Valentina Iori, Donatella Capitani, Luisa Mannina, Stéphane Viel, Annalaura Segre

Affiliation

¹ Istituto di Biologia Agroambientale e Forestale, CNR, Area della Ricerca di Roma, I-00016 Monterotondo Scalo, Rome, Italy.

PMID: 15063201
DOI: 10.1016/j.carres.2004.02.007

Abstract

A high-field NMR study of the polysaccharidic fraction extracted from Lentinula edodes mycelium grown on olive mill waste waters is reported. Diffusion-ordered NMR spectroscopy (DOSY) was applied to the polysaccharidic fraction. The results showed the presence of two polysaccharides of different sizes, whose structures were revealed using one- and two-dimensional NMR techniques. These two polysaccharides were identified as xylan and lentinan.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Carbon / chemistry
Chromatography, Gas
Chromatography, Gel
Hydrogen-Ion Concentration
Lentinan / chemistry
Lentinula / metabolism*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Olea
Polysaccharides / chemistry*
Protons
Temperature
Time Factors
Xylans / chemistry

Substances

Polysaccharides
Protons
Xylans
Lentinan
Carbon