Diminished expression of sodium transporters in the ureteral obstructed kidney in rats

Nephron Exp Nephrol. 2004;96(3):e67-76. doi: 10.1159/000076748.

Abstract

Background/aims: Whether the postobstructive natriuresis and diuresis is related with an altered regulation of sodium transporters in the kidney was examined.

Methods: Male Sprague-Dawley rats underwent either bilateral (BUO) or unilateral obstruction (UUO) of the proximal ureters for 24 h. The expression of Na,K-ATPase, type-3 sodium-hydrogen exchanger (NHE3), type-1 bumetanide-sensitive sodium cotransporter (BSC1), and thiazide-sensitive sodium cotransporter (TSC) proteins was determined in the obstructed kidney by Western blot analysis and immunohistochemistry. Catalytic activity of Na,K-ATPase was also determined.

Results: The expression of alpha1 and beta1 subunit proteins and the catalytic activity of Na,K-ATPase were significantly decreased in the obstructed kidney in BUO. The expressions of NHE3, BSC1 and TSC proteins were also significantly decreased. Immunohistochemistry confirmed the downregulation of these sodium transporters in the obstructed kidney. In UUO, the expression of sodium transporters was similarly decreased in the obstructed kidney.

Conclusion: The postobstructive natriuresis and diuresis may in part be accounted for by a reduced abundance of sodium transporters in the kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / immunology
  • Carrier Proteins / metabolism
  • Diuresis
  • Immunohistochemistry
  • Ion Pumps / metabolism*
  • Kidney / metabolism*
  • Ligation
  • Male
  • Natriuresis*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Drug / immunology
  • Receptors, Drug / metabolism
  • Sodium Chloride Symporters
  • Sodium-Hydrogen Exchanger 3
  • Sodium-Hydrogen Exchangers / immunology
  • Sodium-Hydrogen Exchangers / metabolism
  • Sodium-Potassium-Chloride Symporters / immunology
  • Sodium-Potassium-Chloride Symporters / metabolism
  • Sodium-Potassium-Exchanging ATPase / immunology
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Solute Carrier Family 12, Member 1
  • Solute Carrier Family 12, Member 3
  • Symporters*
  • Ureter / surgery

Substances

  • Carrier Proteins
  • Ion Pumps
  • Receptors, Drug
  • Slc12a1 protein, rat
  • Slc12a3 protein, rat
  • Slc9a3 protein, rat
  • Sodium Chloride Symporters
  • Sodium-Hydrogen Exchanger 3
  • Sodium-Hydrogen Exchangers
  • Sodium-Potassium-Chloride Symporters
  • Solute Carrier Family 12, Member 1
  • Solute Carrier Family 12, Member 3
  • Symporters
  • thiazide receptor
  • Sodium-Potassium-Exchanging ATPase