Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers

Daniel A Di Giusto; Wjatschesslaw A Wlassoff; Susanne Giesebrecht; J Justin Gooding; Garry C King

doi:10.1021/ja0319036

Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers

J Am Chem Soc. 2004 Apr 7;126(13):4120-1. doi: 10.1021/ja0319036.

Authors

Daniel A Di Giusto¹, Wjatschesslaw A Wlassoff, Susanne Giesebrecht, J Justin Gooding, Garry C King

Affiliation

¹ School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia.

PMID: 15053597
DOI: 10.1021/ja0319036

Abstract

Electroactive nucleoside triphosphates ("electrotides") have been incorporated into primers by DNA polymerase and detected on oligonucleotide surface-assembled monolayers. Four electrotides bearing three different electroactive moieties-ferrocene, vinylferrocene, and anthraquinone-are detected in four alternative formats.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / analysis*
DNA / chemistry
DNA / metabolism
DNA Primers* / chemistry
DNA Primers* / metabolism
DNA-Directed DNA Polymerase / metabolism
Electrochemistry / methods*

Substances

DNA Primers
DNA
DNA-Directed DNA Polymerase