The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata

Marcelo Cairrão Araujo Rodrigues; Renato Guizzo; Leonardo Gobbo-Neto; Richard John Ward; Norberto Peporine Lopes; Wagner Ferreira dos Santos

doi:10.1016/j.toxicon.2004.01.009

The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata

Toxicon. 2004 Mar 15;43(4):375-83. doi: 10.1016/j.toxicon.2004.01.009.

Authors

Marcelo Cairrão Araujo Rodrigues¹, Renato Guizzo, Leonardo Gobbo-Neto, Richard John Ward, Norberto Peporine Lopes, Wagner Ferreira dos Santos

Affiliation

¹ Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto da, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, 14049-901 São Paulo, Brazil.

PMID: 15051400
DOI: 10.1016/j.toxicon.2004.01.009

Abstract

Previous work has shown that the crude venom of Parawixia bistriata induces convulsive seizures in rats after intracerebroventricular injection. In this work, the isolation of a bioactive fraction with ultraviolet absorption characteristics of nucleic acid and trace protein or amino acid content is described. NMR analysis demonstrated that the major component of the active fraction is the nucleoside inosine. An analogue of this component (inosine 5'-monophosphate) induced a delayed paralysis effect in termites.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Assay
Inosine / chemistry
Inosine Monophosphate / chemistry*
Inosine Monophosphate / isolation & purification
Inosine Monophosphate / toxicity*
Isoptera / drug effects*
Isoptera / physiology
Magnetic Resonance Spectroscopy
Male
Rats
Rats, Wistar
Spider Venoms / chemistry*
Spider Venoms / isolation & purification
Spider Venoms / toxicity*
Spiders / chemistry*

Substances

Spider Venoms
Inosine Monophosphate
Inosine