Detection of multiple beta-casein (CASB) alleles by amplification created restriction sites (ACRS)

Anim Genet. 1992;23(4):333-8. doi: 10.1111/j.1365-2052.1992.tb00155.x.

Abstract

Direct sequencing of polymerase chain reaction (PCR) amplified DNA has been used to detect the DNA sequences for bovine beta-casein (CASB) A3 and B variants. Based on these sequences we have designed primers which create allele-specific restriction sites in the PCR product. Restriction analysis of PCR product generated in one reaction enable us to identify the A1, A2, A3 and B alleles of CASB rapidly without the use of radioactivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Animals
  • Base Sequence
  • Caseins / genetics*
  • Cattle
  • Gene Amplification*
  • Genetic Variation
  • Male
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligonucleotides
  • Polymerase Chain Reaction
  • Restriction Mapping

Substances

  • Caseins
  • Oligonucleotides