[Effect of mercuric chloride and methylmercury on photosynthetic activity of the diatom Thalassiosira weissflogii by a fluorescent method]

Biofizika. 2004 Jan-Feb;49(1):72-8.
[Article in Russian]

Abstract

It was shown by the pulse-amplitude modulation fluorescent method that, at a weak illumination (6 microE m-2.s-1), methylmercury at a concentration of 10(-6)-10(-7) M decreases the photochemical activity of the reaction centers of photosystem II in cells of microalgae Thalassiosira weissflogii after a prolonged lag phase. Cells resistant to methylmercury at these low concentrations were detected by the microfluorimetric method. Chloride mercury decreased the activity of photosystem II of the algae only when at higher concentrations. Both toxicants at a concentration of 10(-6) M decreased the rate of recovery of photoinduced damage of centers of photosystem II and led to an increase in the energization component of nonphotochemical fluorescence quenching. These results indicate that the complex of fluorescent methods can be used to monitor early changes in the photosynthetic apparatus of algae in response to the toxic action of heavy metals.

Publication types

  • English Abstract

MeSH terms

  • Diatoms / drug effects*
  • Diatoms / physiology
  • Dose-Response Relationship, Drug
  • Mercuric Chloride / pharmacology*
  • Methylmercury Compounds / pharmacology*
  • Photosynthesis / drug effects*
  • Spectrometry, Fluorescence / methods*

Substances

  • Methylmercury Compounds
  • Mercuric Chloride