A streptavidin-biotin-based microarray platform for immunoassays

Methods Mol Biol. 2004:264:73-83. doi: 10.1385/1-59259-759-9:073.

Abstract

The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / metabolism
  • Biotin / metabolism*
  • Gold / metabolism
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods*
  • Immunoglobulin Fragments / metabolism
  • Immunoglobulin M / blood
  • Lyme Disease / diagnosis
  • Lyme Disease / immunology
  • Peptides / metabolism
  • Protein Array Analysis / methods*
  • Reproducibility of Results
  • Streptavidin / metabolism*

Substances

  • Antigens
  • Immunoglobulin Fragments
  • Immunoglobulin M
  • Peptides
  • Biotin
  • Gold
  • Streptavidin