Abstract
The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens / metabolism
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Biotin / metabolism*
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Gold / metabolism
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Humans
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Immunoassay / instrumentation
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Immunoassay / methods*
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Immunoglobulin Fragments / metabolism
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Immunoglobulin M / blood
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Lyme Disease / diagnosis
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Lyme Disease / immunology
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Peptides / metabolism
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Protein Array Analysis / methods*
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Reproducibility of Results
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Streptavidin / metabolism*
Substances
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Antigens
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Immunoglobulin Fragments
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Immunoglobulin M
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Peptides
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Biotin
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Gold
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Streptavidin