Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

Tatsuhiko Sonoda; Syuhei Shigaki; Takeyuki Nagashima; Osamu Okitsu; Yasuhiro Kita; Masaharu Murata; Yoshiki Katayama

doi:10.1016/j.bmcl.2003.12.022

Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

Bioorg Med Chem Lett. 2004 Feb 23;14(4):847-50. doi: 10.1016/j.bmcl.2003.12.022.

Authors

Tatsuhiko Sonoda¹, Syuhei Shigaki, Takeyuki Nagashima, Osamu Okitsu, Yasuhiro Kita, Masaharu Murata, Yoshiki Katayama

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

PMID: 15012979
DOI: 10.1016/j.bmcl.2003.12.022

Abstract

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using (32)P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry / methods
Peptides / chemistry*
Peptides / metabolism*
Protein Kinases / analysis*
Protein Kinases / chemistry
Protein Kinases / metabolism*
Substrate Specificity

Substances

Peptides
Protein Kinases