Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E. coli EWH319 strain odc- null mutant

Biotechnol Lett. 2004 Jan;26(1):75-8. doi: 10.1023/b:bile.0000009465.08712.a6.

Abstract

Ornithine decarboxylase from respiratory fungal pathogen, Coccidioides immitis, cloned in the pETCiODC plasmid under control of T7lac promoter, was produced in E. coli BL21(DE3), BL21(DE3)pLysS, BLR(DE3) and EWH319 transformant strains. E. coli BL21(DE3)pLysS-pETCiODC expressed the highest specific activity of ODC, suggesting that this strain could be successfully used for protein structure and drug testing studies.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coccidioides / enzymology*
  • Coccidioides / genetics*
  • Enzyme Activation
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Gene Expression Regulation, Bacterial / physiology
  • Ornithine Decarboxylase / biosynthesis*
  • Ornithine Decarboxylase / genetics*
  • Protein Engineering / methods*
  • Recombinant Proteins / biosynthesis
  • Species Specificity
  • Substrate Specificity
  • Transformation, Bacterial

Substances

  • Recombinant Proteins
  • Ornithine Decarboxylase