Crystallization and preliminary X-ray analysis of GlcNAc alpha 1,4Gal-releasing endo-beta-galactosidase from Clostridium perfringens

Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):537-8. doi: 10.1107/S0907444903028919. Epub 2004 Feb 25.

Abstract

The unique clostridial endo-beta-galactosidase (Endo-beta-Gal(GnGa)) capable of releasing the disaccharide GlcNAc alpha 1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized. The crystal belongs to space group P6(3), with unit-cell parameters a = 160.4, c = 86.1 A. Under cryocooled conditions and using a synchrotron X-ray source, the crystals diffract to 1.82 A resolution. The asymmetric unit contains two or three molecules.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Clostridium perfringens / enzymology*
  • Crystallization
  • Crystallography, X-Ray
  • Disaccharides / metabolism*
  • Gastric Mucosa / cytology
  • Gastric Mucosa / metabolism
  • Glycoside Hydrolases* / chemistry
  • Glycoside Hydrolases* / metabolism
  • Humans
  • Mucins / metabolism
  • Sialoglycoproteins / metabolism

Substances

  • Bacterial Proteins
  • Disaccharides
  • Mucins
  • Sialoglycoproteins
  • Glycoside Hydrolases
  • keratan-sulfate endo-1,4-beta-galactosidase