A novel bridging ligand bdptb(2,2'-bis(5,6-diphenyl-1,2,4-triazin-3-yl)-4,4'-bipyridine) and it's chiral diruthenium(II) complex DeltaDelta- and LambdaLambda-[Ru(bpy)2(bdptb)Ru(bpy)2]4+ (Ru2) have been synthesized and characterized by electrospray mass spectra, 1H NMR, UV/visible and circular dichroism spectra. Binding behavior of these dimeric complexes with calf thymus DNA have been investigated by absorption spectra, viscosity measurements, equilibrium dialysis experiments. The electronic absorption spectra hypochromism at the metal-ligand charge transfer of the DeltaDelta- and LambdaLambda-enantiomer are 26.4%, and 40%, and bathochromism of 13.5, and 14 nm in sequence. Equilibrium dialysis experiments results show also the binding-DNA of LambdaLambda-enantiomer is stronger than DeltaDelta-enantiomer. The increasing amounts of the novel dimeric ruthenium(II) complexes on the relative viscosities of calf thymus DNA is smaller than that of the classic intercalators such as [Ru(bpy)2(dppz)]2+ and larger than that of the non-classic intercalators such as Delta-[Ru(phen)3]2+. The experiments suggest the dimeric ruthenium(II) complex may be bound to DNA by groove binder.