The growth of scions and rootstocks of compatible (Prunus persica L. Batsch cv. Springtime/Prunus cerasifera L. Ehrh. cv. myrobolan P2032) and incompatible (Prunus persica L. Batsch cv. Springtime/Prunus cerasifera L. Ehrh. cv. myrobolan P18) peach/plum grafts were compared. The composition of soluble carbohydrates in phloem and cortical tissues of both peach/plum grafts and ungrafted plums and the translocation of these compounds across the union of grafted plants were examined. Sorbitol and sucrose were the dominant sugars in the phloem and cortical tissues of plum. A cyanogenic glycoside, prunasin, was present in peach tissues in amounts equivalent to those of sorbitol or sucrose, whereas only small amounts of prunasin were detected in plum tissues. The concentration of prunasin was significantly higher in the phloem of the P18 rootstock of the incompatible graft. Sorbitol was the only sugar significantly depleted in rootstock tissues of the incompatible graft when the first foliar symptoms of graft incompatibility became evident. Translocation studies with 1-(14)C-deoxyglucose showed that the relative distribution of radioactivity across the graft union was similar in both compatible and incompatible grafts. However, the total amount of radioactivity translocated across the incompatible graft was less than one-third of that translocated across the compatible graft. The results are consistent with the hypothesis of a progressive poisoning of the root system in the incompatible graft by a compound synthesized in peach foliage. The role of prunasin as a possible candidate is discussed.