New fragmentation mechanisms in matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry of carbohydrates

Emanuela Spina; Luisa Sturiale; Donatella Romeo; Giuseppe Impallomeni; Domenico Garozzo; Dietmar Waidelich; Matthias Glueckmann

doi:10.1002/rcm.1350

New fragmentation mechanisms in matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry of carbohydrates

Rapid Commun Mass Spectrom. 2004;18(4):392-8. doi: 10.1002/rcm.1350.

Authors

Emanuela Spina¹, Luisa Sturiale, Donatella Romeo, Giuseppe Impallomeni, Domenico Garozzo, Dietmar Waidelich, Matthias Glueckmann

Affiliation

¹ CNR-Istituto di Chimica e Tecnologia dei Polimeri, V. le Regina Margherita 6, 95123 Catania, Italy.

PMID: 14966845
DOI: 10.1002/rcm.1350

Abstract

The spectra recorded by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS/MS) of complex carbohydrates from human milk are presented. Besides ions originating from glycosidic cleavages and from sugar ring fragmentations, these spectra show intense peaks that may be assigned to ions produced by three new fragmentation pathways involving a six-atom rearrangement. These ions, together with the A fragments from sugar ring fragmentations, open the possibility of obtaining a complete mapping of the linkage positions present in the carbohydrates investigated by MALDI-TOF/TOF.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Carbohydrates / analysis*
Carbohydrates / chemistry*
Hexoses / chemistry
Molecular Structure
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Carbohydrates
Hexoses