Cottonseed oil has been used as a fuel source either as a blend with diesel in varying proportions or undiluted (100%) in numerous studies evaluating its potential use in internal combustion engines. However, limited research is available on the use of cottonseed oil as a fuel source in a multi-fueled burner similar to those used by cottonseed oil mills and cotton gins in their drying operations. The purpose of this study was to evaluate emissions from five fuel oil treatments while firing a multi-fueled burner in a setup similar to those used for drying operations of both cottonseed oil mills and cotton gins. For each treatment, gaseous emissions were measured while firing the burner at three fuel flow rates. The five fuel oil treatments evaluated were: (1) No. 2 diesel at 28.3 degrees C, (2) prime bleachable summer yellow (PBSY) cottonseed oil at 28.3 degrees C (PBSY-28), (3) crude cottonseed oil at 28.3 degrees C (Crude-28), (4) PBSY at 60 degrees C (PBSY-60), and (5) crude at 60 degrees C (Crude-60). Results indicate that PBSY treatments had the lowest overall emissions of all treatments. The other treatments varied in emission rates based on treatment and fuel flow rate. Preheating the oil to 60 degrees C resulted in higher NO(x) emissions but displayed varying results in regards to CO. The CO emissions for the crude treatments were relatively unaffected by the 60 degrees C preheat temperature whereas the preheated PBSY treatments demonstrated lower CO emissions. Overall, both cottonseed oils performed well in the multi-fueled burner and displayed a promising potential as an alternative fuel source for cottonseed oil mills and cotton gins in their drying operations.