Abstract
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
MeSH terms
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Electrophoresis, Gel, Two-Dimensional
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Fungal Proteins / analysis
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Gene Expression Profiling
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Gene Expression Regulation, Plant
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Medicago / cytology
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Medicago / genetics
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Medicago / metabolism*
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Mycorrhizae / cytology
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Mycorrhizae / genetics
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Mycorrhizae / metabolism*
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Plant Roots / metabolism*
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Proteome / analysis*
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RNA, Messenger / analysis*
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Symbiosis / physiology
Substances
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Fungal Proteins
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Proteome
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RNA, Messenger